Journal: International Journal of Molecular Sciences

Article Title: NLRP3-Mediated Piezo1 Upregulation in ACC Inhibitory Parvalbumin-Expressing Interneurons Is Involved in Pain Processing after Peripheral Nerve Injury

doi: 10.3390/ijms232113035

Figure Lengend Snippet: SNI activates the morphological transformation of microglia into amoeba-like cells. ( A ) Representative immunofluorescence staining image of Iba1-IR (microglial marker, magenta) in the bilateral ACC of sham and SNI groups. Scale bar = 50 μm. ( B ) Comparison of the process lengths and cell body areas of microglia in the bilateral ACC of sham and SNI groups. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001 versus the sham group (unpaired t test). ( C ) Left: representative immunofluorescence staining image showing CD68-IR in the bilateral ACC in both sham and SNI rats. Scale bar = 50 μm. Right: quantification of the CD68 fluorescence signals in both groups. ** p -value < 0.01 versus the sham group (two-way ANOVA). ( D ) White arrow heads indicate the co-localization of CD68-IR (red) with that of vGAT (green, top) and PV (green, below) 7d post SNI by double staining. Scale bar = 25 μm in the top line; 50 μm in the bottom line. Blue fluorescence in (A, C and D) is from DAPI, a nuclear counterstain.

Article Snippet: GraphPad Software was used for multiple t -test or unpaired t -test or one-way ANOVA or two-way ANOVA to determine the differences over different groups where appropriate.

Techniques: Transformation Assay, Immunofluorescence, Staining, Marker, Comparison, Fluorescence, Double Staining

Journal: bioRxiv

Article Title: Fxr signaling and microbial metabolism of bile salts in the zebrafish intestine

doi: 10.1101/2020.12.13.422569

Figure Lengend Snippet: A. LC/MS chromatographs of medium only control (upper), bile salts only control (middle), and Acinetobacter sp. ZOR0008 culture supplemented with bile salts (bottom) under aerobic conditions. The arrowheads indicate the supplemented bile salts and internal control: black arrowhead: 5αCS; white arrowhead: TCA; grey arrowhead: internal standard D4-GCA. The purple arrow indicates the liberated CA resulted from bacterial deconjugation of TCA. Results from other bacterial strains are shown in Fig S3C. B. Imaging and quantification of GFP fluorescence of the ileal region of 7 dpf Tg(-1.7fabp6:GFP) cyp7a1 -/- larvae that were untreated or treated with either 1 mM TCA or CA for 4 days. The zebrafish ileal region is indicated by arrows. Scale bar = 100 μm. C. qRT-PCR comparing the expression of Fxr target genes in 7 dpf wt germ free larvae that were untreated or treated with either 1mM TCA or CA for 4 days. The results are represented as relative expression levels normalized to the housekeeping gene ef1a (Mean±SEM). Statistical significance was calculated by one-way (B) and two-way (C) ANOVA with Turkey’s multiple comparisons test. Shown are representative data from at least three independent experiments.

Article Snippet: For all other experiments, statistical analysis was performed using unpaired t-test, or one-way or two-way ANOVA with Turkey’s multiple comparisons test with GraphPad Prism.

Techniques: Liquid Chromatography with Mass Spectroscopy, Control, Imaging, Fluorescence, Quantitative RT-PCR, Expressing